Cloning, protein expression, polyclonal antibody preparation and use of nuclear factor TDRP1

The invention belongs to the field of biology and particularly relates to the expression, polyclonal antibody preparation and use of a novel nuclear factor TDRP1. The amino acid sequence of the novel nuclear factor TDRP1 is represented by SEQ ID NO.2 and the nucleotide coding sequence of the novel n...

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Bibliographische Detailangaben
Hauptverfasser:	DING QIANG, WANG XUANCHUN, JIANG HAOWEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of biology and particularly relates to the expression, polyclonal antibody preparation and use of a novel nuclear factor TDRP1. The amino acid sequence of the novel nuclear factor TDRP1 is represented by SEQ ID NO.2 and the nucleotide coding sequence of the novel nuclear factor TDRP1 is represented by SEQ ID NO.1. The invention also provides the polyclonal antibody of the novel nuclear factor TDRP1. The polyclonal antibody has high specificity and high titer. The TDRP1 is mainly expressed in spermatocytes and can be expressed in cell nuclei. Researches on mats of different ages show that the expression of the Tdrp1 gene and the protein in the testicle tissues of newborn mats is very low, but with the sexual development of the mats, the expression TDRP1 in the cell nuclei grows more and more. The result of the researches indicates that the TDRP1 is an important nuclear factor for the generation of sperms and testicle development.