Real-time fluorescence RT-PCR detection method for Renibacterium Salmoninarum and kit

The invention establishes a Tag Man real-time fluorescent quantitative PCR for detecting Renibacterium Salmoninarum, and optimizes the reaction conditions. Proved by specificity, sensitivity and clinical application and experiments, the method has strong specificity and high sensitivity, and is appl...

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Bibliographische Detailangaben
Hauptverfasser:	LIU HONG, SHI XIUJIE, HE JUNQIANG, LAN WENSHENG, LIU ZONGXIAO, GAO LONGYING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention establishes a Tag Man real-time fluorescent quantitative PCR for detecting Renibacterium Salmoninarum, and optimizes the reaction conditions. Proved by specificity, sensitivity and clinical application and experiments, the method has strong specificity and high sensitivity, and is applicable to fast diagnosis on the Renibacterium Salmoninarum and port quarantine inspection, therefore, the invention provides the reliable method for the plague investigation on salmonidae fishes as well as the epidemiological studies on Renibacterium Salmoninarum.