Mutant venin fibrinolytic enzyme gene and preparation method thereof

The invention relates to a synthesis method of a mutant venin fibrinolytic enzyme gene, in particular to a full-length target gene segment prepared by the segmented synthesis of target genes and the artificial synthesis of the overlapping polymerase chain reaction (PCR) technology. The amino acid sequence (GenBank No.P28891) which is published by the national center for biotechnology information (NCBI), of the venin Fibrolase protein of the Agkistrodon contortrix is utilized to design 14 segments of amino acid sequences of which average length is 60bp, wherein the sequences are shown in SEQ ID No.3-16; and the extension and amplification of the synthetized segments are performed to obtain the complete target gene. By adopting the preparation method in the invention, the coding genes of Alfimeprase is easier to obtain, the complicated step that mRNA is converted to cDNA through reverse transcription is eliminated and the corresponding mutant sites can be designed according to needs.