Separation preparation method for high-purity agarotetrol and 4'-methoxy agarotetrol

The invention discloses a high-speed counter-current chromatography separation preparation method for high-purity agarotetrol and 4'-methoxy agarotetrol monomers. The method comprises the following steps of: preparing a crude agilawood extract serving as a sample introduction substance; prepari...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ZHENG KEMIN, ZHANG JIQUAN, LIU WEN, DU CHENJIE, SHEN PINGRANG
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a high-speed counter-current chromatography separation preparation method for high-purity agarotetrol and 4'-methoxy agarotetrol monomers. The method comprises the following steps of: preparing a crude agilawood extract serving as a sample introduction substance; preparing a two-phase solvent system of a stationary phase and a mobile phase; filling the stationary phase in a counter-current chromatograph separation column; rotating a main machine, and pumping the mobile phase into the column; introducing a sample by using a sample introduction valve; and receiving target components according to ultraviolet detection absorption peaks. The solvent system consists of four components (A, B, C and D), wherein the component A is normal paraffin, cycloparaffin or halogenated hydrocarbon; the component B is halogenated hydrocarbon; the component C is fatty alcohol or fatty ketone; and the component D is water. The upper phase is the stationary phase, and the lower phaseis the mobile phase. By t