Reagent assisting in identifying sowbane mosaic virus (SoMV) and application thereof

The invention aims to provide a reagent assisting in identifying a sowbane mosaic virus and application thereof. The reagent provided by the invention comprises a specific primer consisting of deoxyribonucleic acid (DNA) shown as a sequence 1 in a sequence table, DNA shown as a sequence 2 in the seq...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	YANG YIE, SU ZHIPING, GENG JINPEI, WANG ZHEN, SHAO LIHONG, MIU YUGANG, FANG SHAOQING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention aims to provide a reagent assisting in identifying a sowbane mosaic virus and application thereof. The reagent provided by the invention comprises a specific primer consisting of deoxyribonucleic acid (DNA) shown as a sequence 1 in a sequence table, DNA shown as a sequence 2 in the sequence table and DNA shown as a sequence 3 in the sequence table. The reagent can also comprise a specific probe of which the nucleotide sequence is shown as a sequence 4 in the sequence table. The sowbane mosaic virus is an important quarantine harmful pest in China. A method for detecting the SoMVby a nested-RT-Realtime polymerase chain reaction (PCR) is established by three nested PCR primers and a TagMan probe. In the method, a nested PCR and real-time fluorescent PCR technology are combined organically; two sets of PCR systems which consist of three primers and a probe are verified mutually, so that the accuracy of a result is enhanced effectively; and detection sensitivity is enhancedeffectively by the real-ti