Method for manufacturing rotary type sensor used for rapidly, sensitively and specifically detecting trace small RNAs

The invention relates to the technical field of method for manufacturing rotary type sensor used for rapidly, sensitively and specifically detecting trace small RNAs, belongs to the technical field of biosensor, in particular relates to establishment and application of a novel rotary type sensor detection method. The invention has the characteristic that sensitivity and specificity when detecting small RNA with low abundance in cell, tissue, body fluid and blood are rapidly, simply and obviously improved. The invention provides a method for manufacturing novel rotary type small RNAs sensor, used for screening, detecting and comparing expression of endogenous small RNA (such as shRNA, miRNA, piRNA, siRNA and the like) and improving the existing chromatophore purification, preparation and fluorescence labelling, design of probe and sensor assembling and preparation technique of related chip. In the invention, FoF1-ATPase has double actions of biosensor and molecular motor. In the detection process, a probe with