Nucleic acid composition for detecting Datura ferox and application thereof

The invention provides a nucleic acid composition for detecting Datura ferox, which comprises a nucleic acid primer F shown as SEQ ID No. 1, a nucleic acid primer R shown as SEQ ID No. 2 and a nucleic acid molecular probe P shown as SEQ ID No. 3 or a nucleic acid chain which is fully complementary w...

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Bibliographische Detailangaben
Hauptverfasser:	XUE HUAJIE, WEI SHASHA, FU YINING, YI JIANPING, YIN LIPING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a nucleic acid composition for detecting Datura ferox, which comprises a nucleic acid primer F shown as SEQ ID No. 1, a nucleic acid primer R shown as SEQ ID No. 2 and a nucleic acid molecular probe P shown as SEQ ID No. 3 or a nucleic acid chain which is fully complementary with the nucleic acid primer F, the nucleic acid primer R and the nucleic acid molecular probe P. In the invention, by adopting a real-time fluorescent polymerase chain reaction (PCR) detection technology, the nucleic acid composition of the invention can complete detection within half of a working day, thus greatly shortening detection time and obviously improving detection sensitivity. The nucleic acid composition can be applied to the fields such as port inspection and quarantine, agricultural production, plant protection and the like, thus achieving the purpose of rapid and accurate detection and identification.