Novel bio-barcode detection method for ricin

The invention discloses a high-sensitivity novel bio-barcode detection method for ricin, which is used for the detection of ricin protein. According to the novel bio-barcode detection method, a marked bar code DNA strand is improved to be a single strand on the basis of the conventional bio-barcode...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG JINGANG, JIA MINGXIAN, YIN HUIQIONG, YANG SHU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a high-sensitivity novel bio-barcode detection method for ricin, which is used for the detection of ricin protein. According to the novel bio-barcode detection method, a marked bar code DNA strand is improved to be a single strand on the basis of the conventional bio-barcode detection method; the marked bar code DNA strand is prolonged until the marked bar code DNA strand can be detected by a fluorescent quantitative PCR/TaqMan fluorescent probe; and a bar code DNA strand in a sandwich reaction compound does not need to be dissociated and is directly used for performing qualitative detection on the bar code RNA strand in the sandwich reaction compound by a TaqMan fluorescent probe-based real-time fluorescent quantitative PCR method or the conventional PCR method. The method has great practical significance for the high sensitivity detection of the ricin protein and has wide application prospect.