Staphylococcus aureus enterotoxin gene PCR (Polymerase Chain Reaction) parting detection kit and method

The invention relates to a gene parting detection kit for detecting staphylococcus aureus enterotoxin SEA, SEB, SEC and SEE by a PCR (Polymerase Chain Reaction) method as well as a method thereof, belonging to the field of biological detection reagents. The kit comprises amplification reaction liquid, parting reaction liquids A, B, C and E, Taq enzyme, ddH2o, positive standard product of SEA, SEB, SEC and SEE, wherein the parting reaction liquid A contains SEA primers: SEA-F and SEA-R; the parting reaction liquid B contains SEB primers: SEB-F and SEB-R; the parting reaction liquid C contains SEC primers: SEC-F and SEC-R; the parting reaction liquid E contains SEE primers: SEE-F and SEE-R. The detection method comprises the steps of: extracting bacteria DNA, performing parting detection on the enterotoxin SEA, SEB, SEC and SEE genes by using the parting primers designed in the invention under the same PCR instrument and the same condition, and displaying the result via electrophoresis. The detection is rapid,