System and method for improved processing of nucleic acids for production of sequencable libraries

An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site a...

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Bibliographische Detailangaben
Hauptverfasser:	EGHOLM MICHAEL, GODWIN BRIAN C, JEDDELOH JEFFREY, HUTCHISON STEPHEN KYLE, BRAVERMAN MICHAEL S, SIMONS JAN FREDRICK, RONAN MICHAEL TODD, ALBERT THOMAS, RICHES DAVID RODERICK, PALMER MELINDA D, KITZMAN JACOB, FERRERI GIANNI CALOGERO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	An embodiment of an adaptor element for efficient target processing is described that comprises a semi-complementary double stranded nucleic acid adaptor comprising a non-complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complementary region comprises a sequencing primer site and one or more inosine species.