Recombinant TAT-XIAP fusion protein

The invention belongs to the technical field of gene engineering, and particularly relates to a fusion protein formed by fusing a protein transduction domain (PTD) coded by TAT (trans-activator of transcription) genes of HIV (human immunodeficiency viruses) and an X-linked inhibitor of apoptosis pro...

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Bibliographische Detailangaben
Hauptverfasser: JIANG CHANGWEN, LIU MANJUN, LIU YUANJIE, ZHOU SI, ZHANG XIAOHONG, LIN JING, FAN CAIWEN, LIU JING, XIA CHUNBO, QIN XIAOYUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene engineering, and particularly relates to a fusion protein formed by fusing a protein transduction domain (PTD) coded by TAT (trans-activator of transcription) genes of HIV (human immunodeficiency viruses) and an X-linked inhibitor of apoptosis protein (XIAP) nucleotide sequence. The amino acid sequence of the fusion protein is expressed as SEQ ID No: 1. Compared with the prior art, the original XIAP genes with nucleotide sequence expressed as SEQ ID No: 3 are optimized by using escherichia coli preferred codons so that the original XIAP genes are efficiently expressed in escherichia coli, the defect that the XIAP cannot stride the blood brain barrier or enter the brain to play a role in apoptosis resistance is made up by using the transduction capability of the PTD coded by the TAT genes of the HIV so that the final expression product of the genes can enter the brain tissue cells to play a role in cell apoptosis resistance, and the fusion protein is used fo