Duck-origin coronavirus N protein gene, clone method and application thereof

The invention relates to a N protein gene of duck-origin coronavirus ZZ2004, and a clone method and application thereof. The N protein gene sequence has the overall length of 1230 bp, and codes N proteins of 409 amino acid residues. The virus sequence with higher homology is found through random PCR...

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Bibliographische Detailangaben
Hauptverfasser:	WANG SANHU, WANG ZILIANG, ZHAO SHUQIU, WANG XIANWEN, YAO SIXIN, LIU JINHUA, WANG LIRONG, WANG QING, ZHAO KUN, LIU XINGYOU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a N protein gene of duck-origin coronavirus ZZ2004, and a clone method and application thereof. The N protein gene sequence has the overall length of 1230 bp, and codes N proteins of 409 amino acid residues. The virus sequence with higher homology is found through random PCR, a pair of specific primers is designed by using the sequence as the template and the corresponding fragments are amplified, and then the fragments are respectively connected with a pMD18-T carrier to clone the N protein gene of the virus. Due to that the N gene is an important immune gene and has important effect in the aspects of cellular immunity, humoral immunity and local immunity. The research of the N gene of the invention favors the discovery of the IBV heteromorphosis regularity, the distinguishing of the different serotypes and the further development of novel genetic engineering vaccine, and plays important role in the effective prevention of the IBV.