Multiple PCR method for rapidly identifying four varieties of sea cucumbers
The invention relates to a multiple PCR method for rapidly identifying four varieties of sea cucumbers and belongs to the field of molecular biology. The method adopts mitochondria CO I genes of four varieties of sea cucumbers as target DNA, and by sequence comparison, species specificity primers of...
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| creator | ZUO TAO XUE YONG LI ZHAOJIE XUE CHANGHU TANG QINGJUAN LU YINGCHUN DUAN GAOFEI |
| description | The invention relates to a multiple PCR method for rapidly identifying four varieties of sea cucumbers and belongs to the field of molecular biology. The method adopts mitochondria CO I genes of four varieties of sea cucumbers as target DNA, and by sequence comparison, species specificity primers of the four varieties of sea cucumbers are designed respectively. The CO I genes of the sea cucumbersare extracted, multiple-PCR detection is carried out, thus judging the varieties of the sea cucumbers according to the molecular weight of PCR products. After the multiple-PCR detection is carried out on apostichopus japonicus, thelenota ananas, California apostichopus japonicus and Iceland sea cucumbers, the molecular weights of characteristic electrophoretic bands are 202bp, 301bp, 261bp and 358bp sequentially. The identification method has simple operation, low cost, short inspection period, good specificity and repeatability and high sensitivity, overcomes the defects of large workload, long consumed time, high co |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Multiple PCR method for rapidly identifying four varieties of sea cucumbers |
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