Multiple PCR method for rapidly identifying four varieties of sea cucumbers

The invention relates to a multiple PCR method for rapidly identifying four varieties of sea cucumbers and belongs to the field of molecular biology. The method adopts mitochondria CO I genes of four varieties of sea cucumbers as target DNA, and by sequence comparison, species specificity primers of...

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Bibliographische Detailangaben
Hauptverfasser: ZUO TAO, XUE YONG, LI ZHAOJIE, XUE CHANGHU, TANG QINGJUAN, LU YINGCHUN, DUAN GAOFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a multiple PCR method for rapidly identifying four varieties of sea cucumbers and belongs to the field of molecular biology. The method adopts mitochondria CO I genes of four varieties of sea cucumbers as target DNA, and by sequence comparison, species specificity primers of the four varieties of sea cucumbers are designed respectively. The CO I genes of the sea cucumbersare extracted, multiple-PCR detection is carried out, thus judging the varieties of the sea cucumbers according to the molecular weight of PCR products. After the multiple-PCR detection is carried out on apostichopus japonicus, thelenota ananas, California apostichopus japonicus and Iceland sea cucumbers, the molecular weights of characteristic electrophoretic bands are 202bp, 301bp, 261bp and 358bp sequentially. The identification method has simple operation, low cost, short inspection period, good specificity and repeatability and high sensitivity, overcomes the defects of large workload, long consumed time, high co