Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof
The invention discloses an enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit which comprises a capture probe, a rfbE gene probe, an HlyAB gene probe, a stx2 gene probe and a PCR (Polymerase Chain Reaction) amplification primer group A or B of a nucleic acid sample,...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | CHEN MING FU WEILING ZHANG BO GAO WEIYIN LUO YANG WAN YINGHUA LUO CAN |
| description | The invention discloses an enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit which comprises a capture probe, a rfbE gene probe, an HlyAB gene probe, a stx2 gene probe and a PCR (Polymerase Chain Reaction) amplification primer group A or B of a nucleic acid sample, wherein the capture probe is fixed on a solid phase carrier; and the rfbE gene probe, the HlyAB gene probe and the stx2 gene probe are used for respectively emitting quantum dot marks with non-overlapped wavelengths by fluorescence. The invention also discloses a detecting method of the kit, which comprises the following steps of: taking the nucleic acid sample which is suspected to be infected by EHEC O157:H7 and carrying out PCR amplification by using the PCR amplification primer group A or B; hybridizing the obtained PCR product with the capture probe and detecting probes of the three quantum dot marks; detecting the corresponding target gene by the fluorescent emission wavelength and the strength of the quantu |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN101935703BB</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN101935703BB</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN101935703BB3</originalsourceid><addsrcrecordid>eNqNirEOgjAUAFkcjPoPL046aCCENDpCaph0cSdN-6CNtA_K6__r4ODodMndrbNZBsZIFj3FaNXgNMgzaBodHGQrmyM8ikpcWwE-jew-gVKEOanAyYMhhqgmZ8Ago2YXBng5BhV-jUe2ZIAtRqR-m616NS64-3KT7W_y2bQnnKjDZVIaA3LX3Iu8uJSVyMu6Lv-a3sVCQi0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof</title><source>esp@cenet</source><creator>CHEN MING ; FU WEILING ; ZHANG BO ; GAO WEIYIN ; LUO YANG ; WAN YINGHUA ; LUO CAN</creator><creatorcontrib>CHEN MING ; FU WEILING ; ZHANG BO ; GAO WEIYIN ; LUO YANG ; WAN YINGHUA ; LUO CAN</creatorcontrib><description>The invention discloses an enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit which comprises a capture probe, a rfbE gene probe, an HlyAB gene probe, a stx2 gene probe and a PCR (Polymerase Chain Reaction) amplification primer group A or B of a nucleic acid sample, wherein the capture probe is fixed on a solid phase carrier; and the rfbE gene probe, the HlyAB gene probe and the stx2 gene probe are used for respectively emitting quantum dot marks with non-overlapped wavelengths by fluorescence. The invention also discloses a detecting method of the kit, which comprises the following steps of: taking the nucleic acid sample which is suspected to be infected by EHEC O157:H7 and carrying out PCR amplification by using the PCR amplification primer group A or B; hybridizing the obtained PCR product with the capture probe and detecting probes of the three quantum dot marks; detecting the corresponding target gene by the fluorescent emission wavelength and the strength of the quantu</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121010&DB=EPODOC&CC=CN&NR=101935703B$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,782,887,25571,76555</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121010&DB=EPODOC&CC=CN&NR=101935703B$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN MING</creatorcontrib><creatorcontrib>FU WEILING</creatorcontrib><creatorcontrib>ZHANG BO</creatorcontrib><creatorcontrib>GAO WEIYIN</creatorcontrib><creatorcontrib>LUO YANG</creatorcontrib><creatorcontrib>WAN YINGHUA</creatorcontrib><creatorcontrib>LUO CAN</creatorcontrib><title>Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof</title><description>The invention discloses an enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit which comprises a capture probe, a rfbE gene probe, an HlyAB gene probe, a stx2 gene probe and a PCR (Polymerase Chain Reaction) amplification primer group A or B of a nucleic acid sample, wherein the capture probe is fixed on a solid phase carrier; and the rfbE gene probe, the HlyAB gene probe and the stx2 gene probe are used for respectively emitting quantum dot marks with non-overlapped wavelengths by fluorescence. The invention also discloses a detecting method of the kit, which comprises the following steps of: taking the nucleic acid sample which is suspected to be infected by EHEC O157:H7 and carrying out PCR amplification by using the PCR amplification primer group A or B; hybridizing the obtained PCR product with the capture probe and detecting probes of the three quantum dot marks; detecting the corresponding target gene by the fluorescent emission wavelength and the strength of the quantu</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEOgjAUAFkcjPoPL046aCCENDpCaph0cSdN-6CNtA_K6__r4ODodMndrbNZBsZIFj3FaNXgNMgzaBodHGQrmyM8ikpcWwE-jew-gVKEOanAyYMhhqgmZ8Ago2YXBng5BhV-jUe2ZIAtRqR-m616NS64-3KT7W_y2bQnnKjDZVIaA3LX3Iu8uJSVyMu6Lv-a3sVCQi0</recordid><startdate>20121010</startdate><enddate>20121010</enddate><creator>CHEN MING</creator><creator>FU WEILING</creator><creator>ZHANG BO</creator><creator>GAO WEIYIN</creator><creator>LUO YANG</creator><creator>WAN YINGHUA</creator><creator>LUO CAN</creator><scope>EVB</scope></search><sort><creationdate>20121010</creationdate><title>Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof</title><author>CHEN MING ; FU WEILING ; ZHANG BO ; GAO WEIYIN ; LUO YANG ; WAN YINGHUA ; LUO CAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN101935703BB3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2012</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHEN MING</creatorcontrib><creatorcontrib>FU WEILING</creatorcontrib><creatorcontrib>ZHANG BO</creatorcontrib><creatorcontrib>GAO WEIYIN</creatorcontrib><creatorcontrib>LUO YANG</creatorcontrib><creatorcontrib>WAN YINGHUA</creatorcontrib><creatorcontrib>LUO CAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHEN MING</au><au>FU WEILING</au><au>ZHANG BO</au><au>GAO WEIYIN</au><au>LUO YANG</au><au>WAN YINGHUA</au><au>LUO CAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof</title><date>2012-10-10</date><risdate>2012</risdate><abstract>The invention discloses an enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit which comprises a capture probe, a rfbE gene probe, an HlyAB gene probe, a stx2 gene probe and a PCR (Polymerase Chain Reaction) amplification primer group A or B of a nucleic acid sample, wherein the capture probe is fixed on a solid phase carrier; and the rfbE gene probe, the HlyAB gene probe and the stx2 gene probe are used for respectively emitting quantum dot marks with non-overlapped wavelengths by fluorescence. The invention also discloses a detecting method of the kit, which comprises the following steps of: taking the nucleic acid sample which is suspected to be infected by EHEC O157:H7 and carrying out PCR amplification by using the PCR amplification primer group A or B; hybridizing the obtained PCR product with the capture probe and detecting probes of the three quantum dot marks; detecting the corresponding target gene by the fluorescent emission wavelength and the strength of the quantu</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN101935703BB |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Enterohemorrhagic E. coli (EHEC) O157:H7 multicolour quantum dot rapid detecting kit and detecting method thereof |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T17%3A47%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=CHEN%20MING&rft.date=2012-10-10&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN101935703BB%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |