Recombinant adenovirus, preparation method and application thereof

The invention provides recombinant adenovirus, comprising: using a mammal preference codon table to optimize and synthesize the gene of chloride ion channel toxin (BmK CT) of the scorpion; cloning to a intermediate vector of the adenovirus to obtain a recombinant plasmid pShuttle-IRES-hrGFP-2-BmK CT...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	WANG WEI, FU YUEJUN, LIANG AIHUA, YANG RENJIA, ZHANG ZHIYUN, SHEN QUAN, CHAI BAOFENG, DU JUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides recombinant adenovirus, comprising: using a mammal preference codon table to optimize and synthesize the gene of chloride ion channel toxin (BmK CT) of the scorpion; cloning to a intermediate vector of the adenovirus to obtain a recombinant plasmid pShuttle-IRES-hrGFP-2-BmK CT comprising the toxin gene; transforming the recombinant plasmid to the escherichia coli BJ5183 having the adenovirus skeleton genome, obtaining pAdEasy-1-BmK CT after homologous recombination; transfecting the adenovirus genome to the AD293 cell, obtaining the recombinant adenovirus particles AdEasy-1-BmK CT containing the gene of chloride ion channel toxin of the scorpion. The chloride ion channel toxin can inhibit the glioma; with the adenovirus as the carrier, the infection efficiency and the action time of the glioma are improved; the recombinant adenovirus has good application prospect in the gene treatment aspect of the glioma.