SSR (Simple Sequence Repeat) molecular marker identification method of goat breeds

The invention discloses an SSR (Simple Sequence Repeat) molecular marker identification method of goat breeds. The method mainly comprises the following steps of: randomly sampling a blood sample from samples and extracting genome DNA (Deoxyribonucleic Acid) by using a resin-type TM (Melting Tempera...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	CHEN SHICHAO, YANG JIAO, LIU ZHIXUE, ZHENG PEIPEI, LUO QIANQIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses an SSR (Simple Sequence Repeat) molecular marker identification method of goat breeds. The method mainly comprises the following steps of: randomly sampling a blood sample from samples and extracting genome DNA (Deoxyribonucleic Acid) by using a resin-type TM (Melting Temperature) genome DNA extraction kit; screening SSR primers and carrying out PCR (Polymerase Chain Reaction) augmentation to different goat DNA templates with each pair of SSR primers; carrying out electrophoresis detection to an augmentation product and screening out primers with amplification result differences for authenticating goat breeds; and calculating allele composition and the frequency thereof by utilizing PopGene32 and accurately authenticating the breeds through analyzing genetic parameters, such as colony heterozygosity, polymorphism information content, genetic distances, and the like. The SSR molecular marker identification method of the goat breeds, which is provided by the invention, is stable and reli