RNA (Ribonucleic Acid) sequencing quality control method and device relating to gene expression

The invention discloses RNA (Ribonucleic Acid) sequencing quality control method and device relating to gene expression. The method comprises the following steps of: respectively carrying out DGE (Digital Gene Expression) analysis and RNA-Seq (Ribonucleic Acid-Sequence) analysis on a sequencing segm...

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Bibliographische Detailangaben
Hauptverfasser:	PENG ZHIYU, HAN ZUJING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING ELECTRIC DIGITAL DATA PROCESSING ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses RNA (Ribonucleic Acid) sequencing quality control method and device relating to gene expression. The method comprises the following steps of: respectively carrying out DGE (Digital Gene Expression) analysis and RNA-Seq (Ribonucleic Acid-Sequence) analysis on a sequencing segment obtained through a sequencing technology; respectively carrying out correlation analysis between a DGE analysis result and a qPCR (quantitative Polymerase Chain Reaction) result and between a transcriptome analysis result and the qPCR result; judging the difference of DGE analysis and the transcriptome analysis in gene expression quantification according to a correlation analysis result and selecting a sequencing analysis mode from the DGE analysis and the transcriptome analysis; and selecting 1M of reads from analysis results acquired from the selected sequencing analysis mode to analyze the sequencing stability of the gene expression. By carrying out the correlation analysis and the comprehensive estimation o