Nucleic acid constant-temperature amplification kit and method for detecting CPTI transgenic crops

The invention discloses a nucleic acid constant-temperature amplification kit and a method for detecting CPTI transgenic crops, and a group of nucleotide sequences for detecting the CPTI transgenic crops, which belong to the field of inspection and quarantine. The group of nucleotide sequences for d...

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Hauptverfasser:	ZHOU QI, ZHANG XIANGDONG, WANG LIN, GAO ZHIQIANG, PU JING, QI WEI, GU QIANG, DUAN XIANGYING, LING FENGJUN, LI DONGYAN, QIAO CAIXIA, LAI PINGAN, LUO YING, ZHANG LIFENG, ZHANG RUIHONG, BAI YADUO, LIU HUI, ZHANG WEI, WU DAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a nucleic acid constant-temperature amplification kit and a method for detecting CPTI transgenic crops, and a group of nucleotide sequences for detecting the CPTI transgenic crops, which belong to the field of inspection and quarantine. The group of nucleotide sequences for detecting the CPTI transgenic crops comprises: 1) 5'-GCTGGTATTTTTCCTTGTAGG-3' (SEQ ID NO:1); 2) 5'-GACACGAGTTCAACCTGAT-3' (SEQ ID NO:2); 3) 5'-CGCTTGAGTCTGAATCATGATGATTTTTTACTACTGCAGCAATGGA-3' (SEQ ID NO:3); 4) 5'-ATGAACCTTCTGAGTCTTCAGAACTTTCTGTATGATGGCATTGAGG-3' (SEQ ID NO:4); 5) 5'-CCGAGGTGGTTCAAGCAA-3' (SEQ ID NO:5); and 6) 5'-TGCGATTCATGCATCTGCT-3' (SEQ ID NO:6). The nucleic acid constant-temperature amplification and nucleic acid test strip-based CPTI transgenic crop detection kit can simply and fast perform result judgment, is applied to the simple and fast detection of the CPTI transgenic crops, and can be used for the import and export commodity identifier reinspection of laboratories of detection institutio