Loop-mediated isothermal amplification (LAMP) detection method of Angiostrongylus cantonensis

The invention relates to the field of biotechnology, in particular to a method for rapidly detecting Angiostrongylus cantonensis by utilizing the loop-mediated isothermal amplification (LAMP) technology for the rapid detection technology of verminosis. In the method, two pairs of LAMP primers (FIP,...

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Hauptverfasser:	ZHUO MINMIN, TONG QUNBO, LOU DI, LU SHAOHONG, ZHANG YI, CHEN RUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of biotechnology, in particular to a method for rapidly detecting Angiostrongylus cantonensis by utilizing the loop-mediated isothermal amplification (LAMP) technology for the rapid detection technology of verminosis. In the method, two pairs of LAMP primers (FIP, BIP, F3 and B3) for detecting Angiostrongylus cantonensis are provided, and an LAMP method for detecting Angiostrongylus cantonensis by utilizing the two pairs of primers is provided. The method comprises preparation of a reaction template, utilization of an LAMP reaction system and an LAMP reaction program and judgment of detection results. The invention has the advantages of simple and convenient operation, low cost and easy observation of results, and is very suitable for on-site rapid detection of Angiostrongylus cantonensis.