Isothermal amplification detection kit for bird flu H5N1 virus and detecting method thereof

The invention relates to an isothermal amplification detection kit for bird flu H5N1 virus and a detecting method thereof. The kit comprises RNA extract, bird flu H5N1 virus nucleic acid isothermal amplification reaction liquid, bird flu H5N1 virus positive control and negative control. The detectio...

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Hauptverfasser: HU LIN, ZHANG QI, WU JIAPING, ZHANG HONG, ZHANG XIAOHANG, WU TINGYING, YAN JUN, ZHOU XIAN, LI BO, HAN XIAOHUI, WANG JIAN, WANG HONGYING, CHENG YUMING, YE WEI, JIAN DAZHAO, MENG CHENGYAN, ZHANG LIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to an isothermal amplification detection kit for bird flu H5N1 virus and a detecting method thereof. The kit comprises RNA extract, bird flu H5N1 virus nucleic acid isothermal amplification reaction liquid, bird flu H5N1 virus positive control and negative control. The detection kit has the advantages of high specificity, high sensitivity, high reaction speed, suitability for sample detection of both high flux and low flux and no need of complicated instrument in the whole reacting process, wherein only 2 hours are required from treating a single sample to finishing detection.