Hemangioma pathotype subgroup J avian leukosis virus gene and construction of infectious clone thereof

The invention discloses a hemangioma pathotype subgroup J avian leukosis virus gene and the construction of infectious clone thereof. A full-length genome sequence of a subgroup J avian leukosis virus of the invention is shown as SEQ ID NO:1. Then, the hemangioma pathotype subgroup J avian leukosis...

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Hauptverfasser: LUO KAIJIAN, XU CHENGGANG, ZHANG HENAN, XIN CHAOAN, CAO WEISHENG, LAI HANZHANG, LIAO MING
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creator LUO KAIJIAN
XU CHENGGANG
ZHANG HENAN
XIN CHAOAN
CAO WEISHENG
LAI HANZHANG
LIAO MING
description The invention discloses a hemangioma pathotype subgroup J avian leukosis virus gene and the construction of infectious clone thereof. A full-length genome sequence of a subgroup J avian leukosis virus of the invention is shown as SEQ ID NO:1. Then, the hemangioma pathotype subgroup J avian leukosis virus gene shown as SEQ ID NO:1 is subjected to amplification by adopting a specific primer; and pBlueskript SK (I) is used as a vector and chick embryo fibroblast is used as a host cell to construct the infectious clone of the hemangioma pathotype subgroup J avian leukosis virus gene so as to lay the foundation for subsequent research on relation among pathogenic mechanism, a gene structure and functions, virus reproduction, genetic variation rules and the like of the hemangioma pathotype subgroup J avian leukosis virus ALV-J.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Hemangioma pathotype subgroup J avian leukosis virus gene and construction of infectious clone thereof
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