Hemangioma pathotype subgroup J avian leukosis virus gene and construction of infectious clone thereof

The invention discloses a hemangioma pathotype subgroup J avian leukosis virus gene and the construction of infectious clone thereof. A full-length genome sequence of a subgroup J avian leukosis virus of the invention is shown as SEQ ID NO:1. Then, the hemangioma pathotype subgroup J avian leukosis...

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Bibliographische Detailangaben
Hauptverfasser:	LUO KAIJIAN, XU CHENGGANG, ZHANG HENAN, XIN CHAOAN, CAO WEISHENG, LAI HANZHANG, LIAO MING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a hemangioma pathotype subgroup J avian leukosis virus gene and the construction of infectious clone thereof. A full-length genome sequence of a subgroup J avian leukosis virus of the invention is shown as SEQ ID NO:1. Then, the hemangioma pathotype subgroup J avian leukosis virus gene shown as SEQ ID NO:1 is subjected to amplification by adopting a specific primer; and pBlueskript SK (I) is used as a vector and chick embryo fibroblast is used as a host cell to construct the infectious clone of the hemangioma pathotype subgroup J avian leukosis virus gene so as to lay the foundation for subsequent research on relation among pathogenic mechanism, a gene structure and functions, virus reproduction, genetic variation rules and the like of the hemangioma pathotype subgroup J avian leukosis virus ALV-J.