Preparation method of agarose gel microfluidic device

The invention relates to the field of making biochips, in particular to a preparation method of an agarose gel microfluidic device. Particularly, the agarose gel microfluidic device is prepared by three steps of assigning microfilaments, pouring and solidifying, and wiredrawing. The agarose gel microfluidic device manufactured by the method has good sealing property, and the recovery rate of liquid is up to 95%. The agarose gel microfluidic device manufactured by the method can generate stable concentration gradients, and is used for observing interaction between cells and biochemical micro-environments, especially the interaction under a flow condition. The method has simple operation, and the microfluidic device can be manufactured in an ordinary lab without expensive precision instruments.