Alpha-galactosidase and expression and purification method thereof

The invention discloses an alpha-galactosidase and an expression and purification method thereof. The alpha-galactosidase is expressed by SEQ ID NO:1 in a sequence table, is obtained by encoding a recombinant genome DNA sequence SEQ ID NO:3 of bacteroides fragilis (Bacteroides fragilis) ATCC25285 an...

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Bibliographische Detailangaben
Hauptverfasser:	TAN YINGXIA, GONG FENG, JIN SIHU, LI SUBO, WANG YINGLI, YV LIXIA, GAO HONGWEI, JI SHOUPING, BAO GUOQIANG, WANG LINGYAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an alpha-galactosidase and an expression and purification method thereof. The alpha-galactosidase is expressed by SEQ ID NO:1 in a sequence table, is obtained by encoding a recombinant genome DNA sequence SEQ ID NO:3 of bacteroides fragilis (Bacteroides fragilis) ATCC25285 and is a protein with effect of converting a red cell blood group of the human body from B-type into O-type and from AB-type into A-type. The alpha-galactosidase of the invention has the advantages of wide application, simple and convenient expression and purification method, low cost, important clinical and scientific research significance and wide market prospect.