Eukaryotic expression vector of human ski gene, preparation method and application thereof

The invention relates to a eukaryotic expression vector of a human ski gene, a preparation method and application thereof. The eukaryotic expression vector of the human ski gene contains a vector pUC118 segment and a human ski gene segment. The invention further relates to the preparation method of the eukaryotic expression vector of the human ski gene, and application thereof in preparing a preparation for promoting tissue repair and wound healing and inhibiting cicatrisation. The eukaryotic expression vector can be prepared into gene injection or the preparation which can be coated on a wound, or the pUC118-Ski gene injection can be injected into skin, or the eukaryotic expression vector and an excipient can be prepared into emulsion, cream, paste and the like and then applied to injury, thus stably expressing Ski with bioactivity in a long term. Therefore, the eukaryotic expression vector has double functions of promoting tissue repair and wound healing and inhibiting cicatrisation.