Method for separating uridylic acid from biocatalytic conversion solution

The invention discloses a method for separating uridylic acid from biocatalytic conversion solution. The method conducts centrifugation, ultrafiltration, and nanofiltration to the biocatalytic conversion solution. Then, the conversion solution is moved to the anion-exchange column for absorption, deionized water washing, inorganic salt solution elution. The solution after elution is then nanofitrated to demineralise, concentrated and dried to obtain high-yielding, high-purity disodium uridine-5'-monophosphate crystal. The total separation yield rate can reach over 90 percent with the purity of 98 percent. The method of the invention lowers the consumption of resin, and the cost, but improves the yielding rate, the separation performance and the purity of the obtained product. The method is suitable for mass production.