Pullorum-typhoid salmonella PCR detection method, nucleic acid and primer therein

Disclosed are pullorum-typhoid salmonella PCR detection method, nucleic acid and primer therein in food safety inspection technical field; the PCR detection method includes the following procedures: amplification primer is designed according to sequence SEQ ID NO:1; DNA of sample is extracted and th...

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Bibliographische Detailangaben
Hauptverfasser:	LIU BIN, SHI CHUNLEI, HE XIAOHUA, SHI XIANMING, DAN XIANLONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are pullorum-typhoid salmonella PCR detection method, nucleic acid and primer therein in food safety inspection technical field; the PCR detection method includes the following procedures: amplification primer is designed according to sequence SEQ ID NO:1; DNA of sample is extracted and the PCR method is carried out for amplification; the amplification product is detected by gel electrophoresis for judging whether the sample contains pullorum-typhoid salmonella; the judging process concretely includes that: if no corresponding single amplification band occurs, the sample contains nopullorum-typhoid salmonella; the invention also relates to a nucleic acid with base sequence shown in SEQ ID NO:1; the invention also relates to a primer with base sequence shown in SEQ ID NO:2 and SEQ ID NO:3. The detection method of the invention is used for detecting the pullorum-typhoid salmonella, has short detection time, low cost, strong practicality, specific detection results and simple result judgment.