Corn silk polysaccharide separation and decoloring method

The invention relates to a corn silk polysaccharide separation and decoloring method, which is characterized in that the method comprises the following steps: crushing corn silks, then mixing the corn silks with water in a mass ratio of 1: 10 to 1: 15, adding cellulase with the corn silk mass of 1.0...

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Bibliographische Detailangaben
Hauptverfasser: GONG ZHAOJIE, LIU HE, HE YUTANG, PAN XIAOMING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a corn silk polysaccharide separation and decoloring method, which is characterized in that the method comprises the following steps: crushing corn silks, then mixing the corn silks with water in a mass ratio of 1: 10 to 1: 15, adding cellulase with the corn silk mass of 1.0-1.5%, and regulating to a 45 DEG C of constant-temperature water bath with the pH value of 4.5-5.5; filtering polysaccharide leaching liquor after enzymolysis, centrifugating, taking liquid supernatant for vacuum concentration, and removing protein; adding powdered activated carbon into a polysaccharide solution after the removal of protein and the 45 DEG C of constant-temperature water bath; after centrifugation, taking the liquid supernatant, utilizing a microporous filter membrane for sucking filtration for one time to three times, and removing the activated carbon residue; adding anhydrous ethanol or methanol into the decolored silk polysaccharide solution, and centrifugating after uniform mixing; drying preci