Method for determining effective component of total glycoside in smilax glabra ethanol water solution extract

The invention relates to a method for determining an effective component of total glycoside in smilax glabra ethanol water solution extract and belongs to the field of pharmaceutical analysis methods. The method adopts a high-efficiency liquid chromatography to establish a fingerprint chromatogram characterized by taking the astilbin chromatographic peak as an internal reference peak, comprising the following steps of (a) putting 1g of smilax glabra total glycoside dry powder into a 25mL volumetric flask, adding methanol for dissolution and diluting the solution to a scale to obtain sample solution; (b) putting astilbin into methanol solution to prepare reference solution with the concentration of 0.1mg of astilbin per ml; (c) including chromatographic conditions: the chromatographic column is octadecylsilyl as filling; the mobile phase: A phase is methanol, and B phase is gradient elution formed by 0.3 percent of acetic acid solution; the flow velocity is 0.8ml/min; the detection wavelength is 323-327nm; the