Bensulfuron-methyl artificial immunogen BE-BSA, preparation thereof and application thereof

The invention relates to bensulfuron-methyl artificial immunogen BE-BSA, a preparation thereof and application thereof. The preparation for a structural formula comprises the following steps: (1) dissolving bensulfuron-methyl in solution of NaOH for reaction, extracting and separating the solution by chloroform, acidifying an aqueous layer, filtering the solution under reduced pressure, and drying the filtrated solution in vacuum to obtain a purified product; and (2) dissolving bensulfuron-methyl hapten, N-hydroxy succinimide (NHS) and N,N'-dicyclohexyl carbimide (DCC) in an organic solvent, stirring and reacting the mixture, and centrifugating the solution and taking supernatant to obtain active ester; and dissolving bovine serum albumin (BSA) in borate buffer solution (pH 8), adding the active ester into solution of protein under strong stirring, dialyzing the solution after reacting, packing the dialyzed product, and preserving the product at the temperature of 20 DEG C below zero. The preparation is simpl