A method for identifying a pork content in a food

In this study, pork-specific real-time PCR assay is developed for Halal authentication. Three species of meat samples are employed, which were pork, beef and chicken. These three type of poultry meat are among the commonly consumed meat in Malaysia and are easily available in the market. DNA from ea...

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Bibliographische Detailangaben
Hauptverfasser:	B. CHE MAN YAAKOB, ABDUL RAHIM RAHA, MUSTAFA SHUHAIMI, AZMI AIDA AZRINA, KHALID FARIHAH LIYANA, SAZILI AWIS QURNI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	In this study, pork-specific real-time PCR assay is developed for Halal authentication. Three species of meat samples are employed, which were pork, beef and chicken. These three type of poultry meat are among the commonly consumed meat in Malaysia and are easily available in the market. DNA from each raw meat sample was successfully extracted using DNeasy TM Blood & Tissue Kit (Qiagen,Hilden,Germany). Concentration of DNA extracted is estimated by UV absorption spectrophotometry using the Biophotometer (Eppendorf AG,Hamburg,Germany) prior to real-time PCR reaction. The annealing temperature for the primers is at 58 DEG C. To verify the specificity of primers designed, reaction is carried out to test the primers against the other two meat samples to detect possible cross-reactions. The reaction only amplified pork DNA at Ct+-22.83. The real-time PC assay described in this paper proved to be very sensitive with a low detection limit when samples were tested. The assay is done by preparing a 10-fold dilution se