Reactive reagent of nucleic acid amplification by chain replacement at room temperature and nucleic acid amplification method at room temperature thereof

The invention discloses a reactive reagent of nucleic acid amplification by chain replacement at room temperature, belonging to the field of molecular biology and comprising the following components: Tricine buffer solution, potassium chloride, magnesium chloride, dithiothreitol, polyethylene glycol (PEG), ATP, dNTPs, phosphocreatine, a primer pair, SSB protein, colon bacillus RecA protein, yeastRad 51 protein and Bsu DNA polymerase. The invention also discloses a nucleic acid amplification method at room temperature. The nucleic acid amplification of high sensitivity for non-instrument dependent single or multi-sequence single tube type amplification is realized at the room temperature under the chain replacement action of the yeast Rad 51 protein and the colon bacillus RecA protein. The method is used for realizing efficient nucleic acid amplification by the chain replacement under the condition of room temperature, has less use time and simple operation, and omits a traditional heat circulation by a PCR. T