Mutagenic screening method of strain mortierella alpina for generating arachidonic acid

The invention discloses a mutagenic screening method of strain mortierella alpina for generating arachidonic acid. After analyzing a microbe glycolytic pathway, a tricarboxylic acid transferring system, a fat acid synthesizing pathway and other related pathways in the process of producing arachidonic acid (AA) by fermenting the mortierella alpina, iodoacetic acid and acetylsalicylic acid are used as screening agents, and by a plurality of steps of activation of seeds, culture of the seeds, pretreatment of a seed solution, mutation of the treated seed solution, primary screening, secondary screening, third screening and the like, a strain with high growth rate of the arachidonic acid, short fermenting period and large increase range of the yield of the arachidonic acid can be obtained. The method of the invention is an ideal rational strain selective breeding technology and enjoys large economic benefits and social benefits and good industrialization prospects.