Culture medium for overcoming variety and genetype restriction in regeneration culture of alfalfa high frequency somatic embryos

The invention discloses a culture medium for overcoming variety and genetype restriction in regeneration culture of alfalfa high frequency somatic embryos. The culture medium comprises seedling growth and acclimatization culture medium, a somatic cell embryo occurrence and induction culture medium, an embryo subgeneration formation culture medium and an embryo maturing and sprouting culture medium. The seedling growth and acclimatization culture medium is half modified MS+20g/L white sugar+0.7% agaragar; the somatic cell embryo occurrence and induction culture medium is modified SH+6-10mg/L2,4-D+0.2-0.5mg/L 6-BA+30g/L cane sugar+0.3% phytagel; the embryo subgeneration formation culture medium is modified SH+2-5 mg/L 2,4-D+0.2-0.5mg/L6-BA+500mg/L hydrolyzed casein+50g/L cane sugar+0.35% phytagel; and the embryo maturing and sprouting culture medium is modified MS+500mg/L hydrolyzed casein+30g/L white sugar+0.7% agaragar. The invention can effectively overcome the variety and genetype restriction in regeneratio