Production method of n-hexadecyl dibasic acid

The invention relates to a production method of n-hexadecyl dibasic acid, belonging to the technical field of chemical engineering. The production method of n-hexadecyl dibasic acid comprises a fermenting conversion step and a product separation and purification step, the method is characterized in that in the fermenting conversion step, a culture medium contains citric acid, ammonia water, coenzyme Q10, biotin, iron ions, copper ions and zinc ions, and in the fermenting process, 1-5g/l of phosphate and 1-8g/l of nitrate need to be supplemented; and the separation and purification step adoptsthe salting out method, the temperature of the culture solution after fermentation is reduced to 15-20 DEG C, and sodium chloride with the weight concentration of 5-30% is directly added to carry outsalting out pretreatment. The production method of the n-hexadecyl dibasic acid is scientific and reasonable and is convenient in operation, and the obtained product has high purity and low nitrogen content, thus the method im