Method for preparing swine fever-pseudorabies bigeminal live vaccine and product thereof

The invention belongs to the technical field of biological products for veterinary use, in particular to a method for preparing a swine fever-pseudorabies bigeminal live vaccine by using passage cells and a product thereof. The method for preparing the swine fever-pseudorabies bigeminal live vaccine by using the passage cells comprises the following steps: (1) culturing a swine fever lentogen strain and a pseudorabies lentogen strain by the passage cells; (2) harvesting cell culture venoms prepared in the step (1); and (3) mixing two kinds of cell culture venoms prepared in the step (2) in a ratio of 1:2 to prepare the passage cell swine fever-pseudorabies bigeminal live vaccine through cooling and vacuum drying. The invention also provides the swine fever-pseudorabies bigeminal live vaccine prepared by the preparation method. The swine fever-pseudorabies bigeminal live vaccine can be used for immunization, can reduce the workload of the immunization, reduce the number of immunization times, avoid immunologic