Method for extracting monensin sodium by methanol extraction method

The invention provides a method for extracting monensin sodium by a methanol extraction method, belongs to the field of biopharmaceuticals and engineering, and is mainly used for producing a monensin pure substance (sodium salt mode) by a fermentation method. The method comprises the following steps...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WANG JIAN, SONG XUENING, SI SHUJIAN, WEI XIANGCHUN, LIU HUANQIN, WANG JINGGUANG, LI XIULEI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention provides a method for extracting monensin sodium by a methanol extraction method, belongs to the field of biopharmaceuticals and engineering, and is mainly used for producing a monensin pure substance (sodium salt mode) by a fermentation method. The method comprises the following steps: culturing strains in a sand soil tube; culturing strains on an inclined surface; preparing first order seed culture solution and second order seed culture solution; preparing fermentation liquor; making a filter cake; dissolving and releasing the filter cake; extracting the filter cake; and decoloring the filter cake. The method is characterized in that the process of extraction adopts mycelium with moisture content of between 20 and 30 percent, adds 5 to 8 times of methanol of which the concentration is between 60 and 90 percent to the mycelium according to weight ratio, adjusts the temperature at between 15 and 35 DEG C, and stirs the mixture for 30 to 40 minutes to ensure that the mixture is sufficiently disso