Method for increasing silkworm rhabdovirus system expression foreign gene efficiency by using coenospecies female silkworm neuter clone line with naked pupa characteristic

The invention discloses a method for effectively increasing foreign gene efficiency expressed by using a rhabdovirus system by using a coenospecies female silkworm neuter clone line (offspring are female completely) as a host, and the coenospecies female silkworm neuter clone line has a naked pupa c...

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Bibliographische Detailangaben
Hauptverfasser: ZHU XINRONG, HU GUIYAN, MENG ZHIQI, WANG YONGQIANG, LIU XINJU, ZHANG JINWEI, ZHOU WENLIN, HE XIULING, YAO LUSONG, DING NONG, LIU PEIGANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for effectively increasing foreign gene efficiency expressed by using a rhabdovirus system by using a coenospecies female silkworm neuter clone line (offspring are female completely) as a host, and the coenospecies female silkworm neuter clone line has a naked pupa characteristic and is suitable for genetic engineering operation. In the method, silkworm coenospecies which is popularized and applied in production is used as a material, a female silkworm neuter clone technology is used to select individual seed reserving subculture with large pupa body, strong life, high incidence rate and high hatching rate, the coenospecies female silkworm neuter clone line with large pupa body and strong life is cultured by individual and interbatch selection for many years in many generations, and the neuter clone incidence rate and the hatching rate respectively reach above 90 percent and 80 percent. On the basis of the method, the coenospecies female silkworm neuterclone line is hybridized