Method for preparing flavonoid compound by guava cell culture method

The invention relates to a method for preparing a flavonoid compound by a guava cell culture method, which comprises the following steps: firstly, preparing aseptic seedlings by using guava seeds; inoculating the aseptic seedlings on a solid substrate (I) by using the aseptic seedlings as explants;...

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Bibliographische Detailangaben
Hauptverfasser: CHENG JIANGHUA, DING ZHIEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for preparing a flavonoid compound by a guava cell culture method, which comprises the following steps: firstly, preparing aseptic seedlings by using guava seeds; inoculating the aseptic seedlings on a solid substrate (I) by using the aseptic seedlings as explants; culturing callus at 24-26 DEG C; inoculating the callus in a liquid substrate (II) to carry out seed culture and production culture; separating and purifying to obtain general flavone. The substrate (I) is formed by adding 0.5-2.0 mg/L of 2,-4D and 0.0-0.5mg/L of KT, and a pH value is from 5.5 to 5.7; the substrate (II) is an MS substrate without agaragar, and a pH value is from 5.4 to 5.6; the total flavone content in a culture reaches 8.31 percent and is further higher than the flavone content in guava.