Methoxypolyethylene glycol-modified arginine deiminase, preparation thereof and use thereof

The invention discloses a methoxypolyethylene glycol-modified arginine deiminase, preparation thereof and use thereof. As a gratuitous substrate is added in a modification reaction to protect active loci, the modified PEG-ADI has higher biological activity compared with the prior EG-ADI modified at...

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Bibliographische Detailangaben
Hauptverfasser:	WEN XIAOFANG, QIU JIWAN, LIANG WEIFENG, WANG YUJIAO, WANG YEFEI, LING JIANQUN, FAN MIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a methoxypolyethylene glycol-modified arginine deiminase, preparation thereof and use thereof. As a gratuitous substrate is added in a modification reaction to protect active loci, the modified PEG-ADI has higher biological activity compared with the prior EG-ADI modified at the same modification rate. In addition, as specific affinity chromatography is used to remove immunogenic PEG-ADI, the finally prepared PEG-ADI has high activity, high immunologic uniformity and no in-vivo immunogenicity, thereby being more safe and effective in clinic.