Elisa reagent for detecting chloramphenicol and method thereof

Elisa reagent for detecting chloramphenicol and method thereof

The invention provides an elisa kit for detecting chloramphenicol, comprising an enzyme yoke plate with an encrusting peridium source, an enzyme-labeling substance, a chloramphenicol specific antibody, a chloramphenicol standard solution, a substrate visualization solution, a stopping solution, a th...

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Hauptverfasser: LI CHENG, TANG JUN, WEN JUNMEI, YANG HONG, NIE JIBIN, YANG ZONGFAN, WU QING, QI XIN
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator LI CHENG
TANG JUN
WEN JUNMEI
YANG HONG
NIE JIBIN
YANG ZONGFAN
WU QING
QI XIN
description The invention provides an elisa kit for detecting chloramphenicol, comprising an enzyme yoke plate with an encrusting peridium source, an enzyme-labeling substance, a chloramphenicol specific antibody, a chloramphenicol standard solution, a substrate visualization solution, a stopping solution, a thickening and washing solution and a thickening double solution; an encrusting chloramphenicol antigen is obtained by coupling a chloramphenicol hapten and bovine gamma globulin by an active ester method, and an antibody is a polyclonal antibody or a monoclonal antibody. The invention also provides a method for detecting the chloramphenicol in animal-derived food, comprising the following steps: sample pre-treatment; detection by reagents in the kit; and detection result analysis. The elisa kit and the detection method are simple and convenient to operate, have low cost and are suitable for screening a great amount of samples to detect the chloramphenicol in the animal-derived food.
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The invention also provides a method for detecting the chloramphenicol in animal-derived food, comprising the following steps: sample pre-treatment; detection by reagents in the kit; and detection result analysis. 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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Elisa reagent for detecting chloramphenicol and method thereof
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