Elisa reagent for detecting chloramphenicol and method thereof

The invention provides an elisa kit for detecting chloramphenicol, comprising an enzyme yoke plate with an encrusting peridium source, an enzyme-labeling substance, a chloramphenicol specific antibody, a chloramphenicol standard solution, a substrate visualization solution, a stopping solution, a thickening and washing solution and a thickening double solution; an encrusting chloramphenicol antigen is obtained by coupling a chloramphenicol hapten and bovine gamma globulin by an active ester method, and an antibody is a polyclonal antibody or a monoclonal antibody. The invention also provides a method for detecting the chloramphenicol in animal-derived food, comprising the following steps: sample pre-treatment; detection by reagents in the kit; and detection result analysis. The elisa kit and the detection method are simple and convenient to operate, have low cost and are suitable for screening a great amount of samples to detect the chloramphenicol in the animal-derived food.