Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase

The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature am...

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Hauptverfasser: WEI CHENFEI, WANG QINGSHENG, ZHOU WEIFENG, TAO DINGWEI
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creator WEI CHENFEI
WANG QINGSHENG
ZHOU WEIFENG
TAO DINGWEI
description The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature amylase, which has the preservation number of CCTCC NO: M207193, and is obtained through compound mutation of the prior Bacillus licheniformis system, preservation, rejuvenation and a selective breeding method; and then the Bacillus licheniformis is optimized by a culture medium formulation and optimized by fermenting technological conditions so as to establish the method for producing the acid-proof high-temperature amylase, wherein the method comprises the following steps: air culturing the Bacillus licheniformis for about 72 hours at a temperature of 37 DEG C to reach an enzyme generating peak; and obtaining concentrated enzyme fluid through flocculating treatment, pressure filtration and ultra-filtration concentr
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase
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