Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase

The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature am...

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Hauptverfasser: WEI CHENFEI, WANG QINGSHENG, ZHOU WEIFENG, TAO DINGWEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature amylase, which has the preservation number of CCTCC NO: M207193, and is obtained through compound mutation of the prior Bacillus licheniformis system, preservation, rejuvenation and a selective breeding method; and then the Bacillus licheniformis is optimized by a culture medium formulation and optimized by fermenting technological conditions so as to establish the method for producing the acid-proof high-temperature amylase, wherein the method comprises the following steps: air culturing the Bacillus licheniformis for about 72 hours at a temperature of 37 DEG C to reach an enzyme generating peak; and obtaining concentrated enzyme fluid through flocculating treatment, pressure filtration and ultra-filtration concentr