Novel method for preparing nucleotide by biological phosphorylation technology

The invention discloses a novel method for preparing nucleotide by utilizing a biological phosphoryl technique. In the method, a precursor of purine or pyrimidine nucleotide and phosphate ion are used as substrates, glucose is used as a ribose group donor and energy donor, a permeable microbial cell is used, and a chemical micro molecular effect matter is used to control a cofactor regeneration system, a matter energy coupling regeneration system and the enzymatic activity of a metabolic pathway key enzyme, so that the high-efficiency preparation of the nucleotide is realized. Because the micro molecular chemical effect matter is used to control the metabolic flow, the regeneration and the utilization conditions of the energy and the auxiliary enzyme are improved, the metabolic flux is transferred to the target path, the by products of the metabolism are reduced, and the product yield is improved; and the method for changing the permeability of a cell membrane can speed up the permeation of reaction component