Method for producing recombinant sea cucumber antalzyme and recombinant sea cucumber antalzyme produced thereby

The invention relates to a production method for recombinant holothurian lysozyme and the recombinant holothurian lysozyme produced by the method. The method comprises the following steps: collecting a holothurian lysozyme gene (SEQ ID NO.1) to a eukaryotic expression vector pPIC9K to construct recombinant plasmid, transforming the formed recombinant plasmid into Pichia pastoris SMD 1168, inducing the recombinant plasmid by methanol and expressing the recombinant plasmid to obtain the recombinant lysozyme after purification for cation resin chromatographic column. The method adopts genetically engineered vaccine strains to produce lysozyme, is suitable for large-scale culture and extraction separation, and has the advantages of simple processing technology, simple equipment requirement, short production period, lower cost and no environment pollution. The activity of the prepared recombinant holothurian lysozyme is increased by 30 percent compared with the lysozyme extracted from holothurian tissues; and comp