Rapid diagnosis reagent kit and detection method for pseudomonas aeruginosa gene
The invention discloses a rapid diagnosis kit and a detection method of genes of pseudomonas aeruginosa. The kit consists of two pairs of primers, DNA polymerase, a stabilizing solution, a reaction solution, a sample pre-treatment solution, a color development solution and a positive comparison solu...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a rapid diagnosis kit and a detection method of genes of pseudomonas aeruginosa. The kit consists of two pairs of primers, DNA polymerase, a stabilizing solution, a reaction solution, a sample pre-treatment solution, a color development solution and a positive comparison solution, wherein, the seven solutions are respectively placed in containers. The rapid diagnosis kit of genes of the invention applies six sections and four primers and can judge whether target materials exist only according to the amplification, thus being high in specificity. The rapid diagnosis kit of genes of the invention is high in speed, high in efficiency and sensitivity, can amplify the reaction with only constant temperature, without special reagents or equipment, and is low in detection cost. The rapid diagnosis kit of genes of the invention is simple and convenient in identification. Pyrophosphate ions separated out from the dNTP are combined with Mg in the reaction solution to produce a by-product whi |
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