Bean pod mottle virus fluorescent quantitative RT-PCR kit and detection method thereof

The invention relates to a bean pod mottle virus fluorescence quantitative RT-PCR reagent kit and a detection method thereof. The reagent kit comprises reverse transcriptase, an RNA enzyme inhibitor, hot start Taq DNA polymerase, fluorescence quantitative reaction solution, 2 x Buffer, sterilized double distilled water containing no RNA enzyme and positive contrast. The reagent kit and the detection method thereof use a conservative fragment of coat protein CP gene of bean pod mottle virus as a target, design a specificity primer and a TaqMan-MGB fluorescent probe, utilize the fluorescence quantitative RT-PCR technology for detecting the bean pod mottle virus, have the advantages of high sensitivity, strong specificity, rapidness, simplicity, accuracy and reliability, need no PCR posttreatment step, can effectively prevent cross contamination and false positive, are suitable for entry and exit plant quarantine, species introduction and rapid detection and diagnosis of the bean pod mottle virus in agricultural