Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin
The invention relates to a compatible separating material of lactoferrin in the technical field of biomedicine and a compatible purification method of lactoferrin. The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly,...
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creator | HUANG FEIYUN REN HUIJUAN HUO CHENXI WEN SHENG LI RONGXIU |
description | The invention relates to a compatible separating material of lactoferrin in the technical field of biomedicine and a compatible purification method of lactoferrin. The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly, a basic chromatography medium with amido or a conventional chemical method is used for derivatization to the basic chromatography medium, and the amido is carried for activating reaction with trichloro-triazine; and then the basic chromatography medium reacts with beta-lactamine or 1-aminocyclohexane methanoic acid to synthesize the compatible separating material; step two, biomimetic compatible separating material prepared is used for purifying the lactoferrin. The material and the method canlead to mass production with high-purity lactoferrin, high efficiency and low cost. |
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The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly, a basic chromatography medium with amido or a conventional chemical method is used for derivatization to the basic chromatography medium, and the amido is carried for activating reaction with trichloro-triazine; and then the basic chromatography medium reacts with beta-lactamine or 1-aminocyclohexane methanoic acid to synthesize the compatible separating material; step two, biomimetic compatible separating material prepared is used for purifying the lactoferrin. 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The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly, a basic chromatography medium with amido or a conventional chemical method is used for derivatization to the basic chromatography medium, and the amido is carried for activating reaction with trichloro-triazine; and then the basic chromatography medium reacts with beta-lactamine or 1-aminocyclohexane methanoic acid to synthesize the compatible separating material; step two, biomimetic compatible separating material prepared is used for purifying the lactoferrin. The material and the method canlead to mass production with high-purity lactoferrin, high efficiency and low cost.</abstract><oa>free_for_read</oa></addata></record> |
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title | Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin |
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